Fluorescing Isofunctional Ribonucleosides: Assessing Adenosine Deaminase Activity and Inhibition.
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ABSTRACT:
SUBMITTER: Ludford PT
PROVIDER: S-EPMC6467514 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
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Fluorescing Isofunctional Ribonucleosides: Assessing Adenosine Deaminase Activity and Inhibition.
Ludford Paul T PT Rovira Alexander R AR Fin Andrea A Tor Yitzhak Y
Chembiochem : a European journal of chemical biology 20190221 5
The enzymatic conversion of isothiazolo[4,3-d]pyrimidine-based adenosine (<sup>tz</sup> A) and 2-aminoadenosine (<sup>tz</sup> 2-AA) analogues to the corresponding isothiazolo[4,3-d]pyrimidine-based inosine (<sup>tz</sup> I) and guanosine (<sup>tz</sup> G) derivatives is evaluated and compared to the conversion of native adenosine to inosine. Henri-Michaelis-Menten analyses provides the foundation for a high-throughput screening assay, and the efficacy of the assay is showcased by fluorescence-b ...[more]
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