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Rapid, label-free genetic detection of enteropathogens in stool without genetic isolation or amplification.


ABSTRACT: Current genetic detection methods require gene isolation, gene amplification and detection with a fluorescent-tagged probe. They typically require sophisticated equipment and expensive fluorescent probes, rendering them not widely available for rapid acute infection diagnoses at the point of care to ensure timely treatment of the diseases. Here we report a rapid genetic detection method that can detect the bacterial gene directly from patient stools using a piezoelectric plate sensor (PEPS) in conjunction with a continuous flow system with two temperature zones. With stools spiked with sodium dodecyl sulfate (SDS) in situ bacteria lysing and DNA denaturation occurred in the high-temperature zone whereas in situ specific detection of the denatured DNA by the PEPS occurred in the lower-temperature zone. The outcome was a rapid genetic detection method that directly detected bacterial genes from stool in

SUBMITTER: Han S 

PROVIDER: S-EPMC6469511 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Rapid, label-free genetic detection of enteropathogens in stool without genetic isolation or amplification.

Han Song S   Soylu Mehmet C MC   Kirimli Ceyhun E CE   Wu Wei W   Sen Bhaswati B   Joshi Suresh G SG   Emery Christopher L CL   Au Giang G   Niu Xiaomin X   Hamilton Richard R   Krevolin Kyle K   Shih Wei-Heng WH   Shih Wan Y WY  

Biosensors & bioelectronics 20190121


Current genetic detection methods require gene isolation, gene amplification and detection with a fluorescent-tagged probe. They typically require sophisticated equipment and expensive fluorescent probes, rendering them not widely available for rapid acute infection diagnoses at the point of care to ensure timely treatment of the diseases. Here we report a rapid genetic detection method that can detect the bacterial gene directly from patient stools using a piezoelectric plate sensor (PEPS) in c  ...[more]

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