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Low-coherent optical diffraction tomography by angle-scanning illumination.

ABSTRACT: Temporally low-coherent optical diffraction tomography (ODT) is proposed and demonstrated based on angle-scanning Mach-Zehnder interferometry. Using a digital micromirror device based on diffractive tilting, the full-field interference of incoherent light is successfully maintained during every angle-scanning sequences. Further, current ODT reconstruction principles for temporally incoherent illuminations are thoroughly reviewed and developed. Several limitations of incoherent illumination are also discussed, such as the nondispersive assumption, optical sectioning capacity and illumination angle limitation. Using the proposed setup and reconstruction algorithms, low-coherent ODT imaging of plastic microspheres, human red blood cells and rat pheochromocytoma cells is experimentally demonstrated.

SUBMITTER: Lee K

PROVIDER: S-EPMC6470054 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Low-coherent optical diffraction tomography by angle-scanning illumination.

Lee KyeoReh K Shin Seungwoo S Yaqoob Zahid Z So Peter T C PTC Park YongKeun Y

Journal of biophotonics 20190128 5

Temporally low-coherent optical diffraction tomography (ODT) is proposed and demonstrated based on angle-scanning Mach-Zehnder interferometry. Using a digital micromirror device based on diffractive tilting, the full-field interference of incoherent light is successfully maintained during every angle-scanning sequences. Further, current ODT reconstruction principles for temporally incoherent illuminations are thoroughly reviewed and developed. Several limitations of incoherent illumination are a ...[more]

PMID: 30597743

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