Dataset Information

Identification of Zebrafish Calcium Toolkit Genes and their Expression in the Brain.

ABSTRACT: Zebrafish are well-suited for in vivo calcium imaging because of the transparency of their larvae and the ability to express calcium probes in various cell subtypes. This model organism has been used extensively to study brain development, neuronal function, and network activity. However, only a few studies have investigated calcium homeostasis and signaling in zebrafish neurons, and little is known about the proteins that are involved in these processes. Using bioinformatics analysis and available databases, the present study identified 491 genes of the zebrafish Calcium Toolkit (CaTK). Using RNA-sequencing, we then evaluated the expression of these genes in the adult zebrafish brain and found 380 hits that belonged to the CaTK. Based on quantitative real-time polymerase chain reaction arrays, we estimated the relative mRNA levels in the brain of CaTK genes at two developmental stages. In both 5 dpf larvae and adult zebrafish, the highest relative expression was observed for tmbim4, which encodes a Golgi membrane protein. The present data on CaTK genes will contribute to future applications of zebrafish as a model for in vivo and in vitro studies of Ca²⁺ signaling.

SUBMITTER: Wasilewska I

PROVIDER: S-EPMC6471419 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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Identification of Zebrafish Calcium Toolkit Genes and their Expression in the Brain.

Wasilewska Iga I Gupta Rishikesh Kumar RK Palchevska Oksana O Kuźnicki Jacek J

Genes 20190318 3

Zebrafish are well-suited for in vivo calcium imaging because of the transparency of their larvae and the ability to express calcium probes in various cell subtypes. This model organism has been used extensively to study brain development, neuronal function, and network activity. However, only a few studies have investigated calcium homeostasis and signaling in zebrafish neurons, and little is known about the proteins that are involved in these processes. Using bioinformatics analysis and availa ...[more]

PMID: 30889933

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Dataset Information

Identification of Zebrafish Calcium Toolkit Genes and their Expression in the Brain.

Publications

Identification of Zebrafish Calcium Toolkit Genes and their Expression in the Brain.

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