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Reductive metabolism of the important atmospheric gas isoprene by homoacetogens.

ABSTRACT: Isoprene is the most abundant biogenic volatile organic compound (BVOC) in the Earth's atmosphere and plays important roles in atmospheric chemistry. Despite this, little is known about microbiological processes serving as a terrestrial sink for isoprene. While aerobic isoprene degrading bacteria have been identified, there are no known anaerobic, isoprene-metabolizing organisms. In this study an H₂-consuming homoacetogenic enrichment was shown to utilize 1.6??moles isoprene h^-1 as an electron acceptor in addition to HCO₃^-. The isoprene-reducing community was dominated by Acetobacterium spp. and isoprene was shown to be stoichiometrically reduced to three methylbutene isomers (2-methyl-1-butene (>97%), 3-methyl-1-butene (?2%), 2-methyl-2-butene (?1%). In the presence of isoprene, 40% less acetate was formed suggesting that isoprene reduction is coupled to energy conservation in Acetobacterium spp. This study improves our understanding of linkages and feedbacks between biogeochemistry and terrestrial microbial activity.

SUBMITTER: Kronen M

PROVIDER: S-EPMC6474224 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Reductive metabolism of the important atmospheric gas isoprene by homoacetogens.

Kronen Miriam M Lee Matthew M Jones Zackary L ZL Manefield Michael J MJ

The ISME journal 20190114 5

Isoprene is the most abundant biogenic volatile organic compound (BVOC) in the Earth's atmosphere and plays important roles in atmospheric chemistry. Despite this, little is known about microbiological processes serving as a terrestrial sink for isoprene. While aerobic isoprene degrading bacteria have been identified, there are no known anaerobic, isoprene-metabolizing organisms. In this study an H<sub>2</sub>-consuming homoacetogenic enrichment was shown to utilize 1.6 μmoles isoprene h<sup>-1< ...[more]

PMID: 30643199

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Dataset Information

Reductive metabolism of the important atmospheric gas isoprene by homoacetogens.

Publications

Reductive metabolism of the important atmospheric gas isoprene by homoacetogens.

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