Unknown

Dataset Information

0

Histone 2B monoubiquitination complex integrates transcript elongation with RNA processing at circadian clock and flowering regulators.


ABSTRACT: HISTONE MONOUBIQUITINATION1 (HUB1) and its paralog HUB2 act in a conserved heterotetrameric complex in the chromatin-mediated transcriptional modulation of developmental programs, such as flowering time, dormancy, and the circadian clock. The KHD1 and SPEN3 proteins were identified as interactors of the HUB1 and HUB2 proteins with in vitro RNA-binding activity. Mutants in SPEN3 and KHD1 had reduced rosette and leaf areas. Strikingly, in spen3 mutants, the flowering time was slightly, but significantly, delayed, as opposed to the early flowering time in the hub1-4 mutant. The mutant phenotypes in biomass and flowering time suggested a deregulation of their respective regulatory genes CIRCADIAN CLOCK-ASSOCIATED1 (CCA1) and FLOWERING LOCUS C (FLC) that are known targets of the HUB1-mediated histone H2B monoubiquitination (H2Bub). Indeed, in the spen3-1 and hub1-4 mutants, the circadian clock period was shortened as observed by luciferase reporter assays, the levels of the CCA1? and CCA1? splice forms were altered, and the CCA1 expression and H2Bub levels were reduced. In the spen3-1 mutant, the delay in flowering time was correlated with an enhanced FLC expression, possibly due to an increased distal versus proximal ratio of its antisense COOLAIR transcript. Together with transcriptomic and double-mutant analyses, our data revealed that the HUB1 interaction with SPEN3 links H2Bub during transcript elongation with pre-mRNA processing at CCA1 Furthermore, the presence of an intact HUB1 at the FLC is required for SPEN3 function in the formation of the FLC-derived antisense COOLAIR transcripts.

SUBMITTER: Woloszynska M 

PROVIDER: S-EPMC6475378 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

Histone 2B monoubiquitination complex integrates transcript elongation with RNA processing at circadian clock and flowering regulators.

Woloszynska Magdalena M   Le Gall Sabine S   Neyt Pia P   Boccardi Tommaso M TM   Grasser Marion M   Längst Gernot G   Aesaert Stijn S   Coussens Griet G   Dhondt Stijn S   Van De Slijke Eveline E   Bruno Leonardo L   Fung-Uceda Jorge J   Mas Paloma P   Van Montagu Marc M   Inzé Dirk D   Himanen Kristiina K   De Jaeger Geert G   Grasser Klaus D KD   Van Lijsebettens Mieke M  

Proceedings of the National Academy of Sciences of the United States of America 20190328 16


HISTONE MONOUBIQUITINATION1 (HUB1) and its paralog HUB2 act in a conserved heterotetrameric complex in the chromatin-mediated transcriptional modulation of developmental programs, such as flowering time, dormancy, and the circadian clock. The KHD1 and SPEN3 proteins were identified as interactors of the HUB1 and HUB2 proteins with in vitro RNA-binding activity. Mutants in <i>SPEN3</i> and <i>KHD1</i> had reduced rosette and leaf areas. Strikingly, in <i>spen3</i> mutants, the flowering time was  ...[more]

Similar Datasets

| S-EPMC4600324 | biostudies-literature
| S-EPMC3378035 | biostudies-literature
| S-EPMC2327256 | biostudies-literature
| S-EPMC5780430 | biostudies-literature
| S-EPMC3204309 | biostudies-literature
| S-EPMC2757846 | biostudies-other
| S-EPMC6003885 | biostudies-literature
| S-EPMC4303289 | biostudies-other
| S-EPMC8557835 | biostudies-literature
| S-EPMC4774988 | biostudies-literature