Role of cytochrome epoxygenase (CYP2J2) in the pathophysiology of coronary artery disease in South Indian population.

ABSTRACT:

Background

The cytochrome P-450 2J2 (CYP2J2) is known to be one of the major enzymes of epoxygenase pathway of arachidonic acid in extrahepatic tissues, which produces series of regioisomeric cis-epoxyeicosatrienoic acids (EETs) such as 5,6-, 8,9-, 11,12-, and 14,15-EETs. In the present study, we analyzed the impact of a genetic variant in CYP2J2 on coronary artery disease (CAD) in the Telangana region of Indian population.

Material and methods

The case-control study consisted of 100 CAD cases and 110 healthy controls. The deoxyribonucleic acid was extracted using the salting out method. Genotyping and gene expression was performed by polymerase chain reaction (PCR)-restriction fragment length polymorphism and real-time-PCR methods.

Results

In the present study, the p

SUBMITTER: Tantray JA 

PROVIDER: S-EPMC6477124 | biostudies-literature | 2019 Jan - Feb

REPOSITORIES: biostudies-literature