Development and characterization of 17 polymorphic microsatellite markers for the reef manta ray (Mobula alfredi).
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ABSTRACT: OBJECTIVE:Limited sample sizes are often a problem for species of conservation concern when using genetic tools to make population assessments. Lack of analytical power from small sample sizes can be compensated for by use of a large marker set. Here we report on development and characterization of 17 novel microsatellite markers for the reef manta ray (Mobula alfredi). RESULTS:Loci were screened on 60 reef manta rays (M. alfredi) sampled from the east coast of Australia. The number of alleles per locus varied from 2 to 13 with observed heterozygosities ranging between 0.300 and 0.917. The development of these 17 additional markers increases the total number of microsatellite markers available for this species to 27.
SUBMITTER: Armstrong AJ
PROVIDER: S-EPMC6477734 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
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