CMY-1/MOX-family AmpC ?-lactamases MOX-1, MOX-2 and MOX-9 were mobilized independently from three Aeromonas species.

ABSTRACT: OBJECTIVES:To investigate the origin of CMY-1/MOX-family ?-lactamases. METHODS:Publicly available genome assemblies were screened for CMY-1/MOX genes. The loci of CMY-1/MOX genes were compared with respect to synteny and nucleotide identity, and subjected to phylogenetic analysis. RESULTS:The chromosomal ampC genes of several Aeromonas species were highly similar to known mobile CMY-1/MOX variants. Annotation and sequence comparison revealed nucleotide identities >98% and conserved syntenies between MOX-1-, MOX-2- and MOX-9-associated mobile sequences and the chromosomal Aeromonas sanarellii, Aeromonas caviae and Aeromonas media ampC loci. Furthermore, the phylogenetic analysis showed that MOX-1, MOX-2 and MOX-9 formed three distinct monophyletic groups with the chromosomal ampC genes of A. sanarellii, A. caviae and A. media, respectively. CONCLUSIONS:Our findings show that three CMY-1/MOX-family ?-lactamases were mobilized independently from three Aeromonas species and hence shine new light on the evolution and emergence of mobile antibiotic resistance genes.

SUBMITTER: Ebmeyer S 

PROVIDER: S-EPMC6477974 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature