Binding studies of a putative C. pseudotuberculosis target protein from Vitamin B₁₂ Metabolism.

ABSTRACT: Vitamin B₁₂ acts as a cofactor for various metabolic reactions important in living organisms. The Vitamin B₁₂ biosynthesis is restricted to prokaryotes, which means, all eukaryotic organisms must acquire this molecule through diet. This study presents the investigation of Vitamin B₁₂ metabolism and the characterization of precorrin-4 C(11)-methyltransferase (CobM), an enzyme involved in the biosynthesis of Vitamin B₁₂ in Corynebacterium pseudotuberculosis. The analysis of the C. pseudotuberculosis genome identified two Vitamin B₁₂-dependent pathways, which can be strongly affected by a disrupted vitamin metabolism. Molecular dynamics, circular dichroism, and NMR-STD experiments identified regions in CobM that undergo conformational cha

SUBMITTER: Peinado RDS 

PROVIDER: S-EPMC6478909 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature