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Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region.


ABSTRACT: The hypothalamus controls essential social behaviors and homeostatic functions. However, the cellular architecture of hypothalamic nuclei-including the molecular identity, spatial organization, and function of distinct cell types-is poorly understood. Here, we developed an imaging-based in situ cell-type identification and mapping method and combined it with single-cell RNA-sequencing to create a molecularly annotated and spatially resolved cell atlas of the mouse hypothalamic preoptic region. We profiled ~1 million cells, identified ~70 neuronal populations characterized by distinct neuromodulatory signatures and spatial organizations, and defined specific neuronal populations activated during social behaviors in male and female mice, providing a high-resolution framework for mechanistic investigation of behavior circuits. The approach described opens a new avenue for the construction of cell atlases in diverse tissues and organisms.

SUBMITTER: Moffitt JR 

PROVIDER: S-EPMC6482113 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region.

Moffitt Jeffrey R JR   Bambah-Mukku Dhananjay D   Eichhorn Stephen W SW   Vaughn Eric E   Shekhar Karthik K   Perez Julio D JD   Rubinstein Nimrod D ND   Hao Junjie J   Regev Aviv A   Dulac Catherine C   Zhuang Xiaowei X  

Science (New York, N.Y.) 20181101 6416


The hypothalamus controls essential social behaviors and homeostatic functions. However, the cellular architecture of hypothalamic nuclei-including the molecular identity, spatial organization, and function of distinct cell types-is poorly understood. Here, we developed an imaging-based in situ cell-type identification and mapping method and combined it with single-cell RNA-sequencing to create a molecularly annotated and spatially resolved cell atlas of the mouse hypothalamic preoptic region. W  ...[more]

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