A fast, reliable and sample-sparing method to identify fibre types of single muscle fibres.
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ABSTRACT:
SUBMITTER: Christiansen D
PROVIDER: S-EPMC6482153 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Publications
A fast, reliable and sample-sparing method to identify fibre types of single muscle fibres.
Christiansen Danny D MacInnis Martin J MJ Zacharewicz Evelyn E Xu Hongyang H Frankish Barnaby P BP Murphy Robyn M RM
Scientific reports 20190424 1
Many skeletal muscle proteins are present in a cell-specific or fibre-type dependent manner. Stimuli such as exercise, aging, and disease have been reported to result in fibre-specific responses in protein abundances. Thus, fibre-type-specific determination of the content of specific proteins provides enhanced mechanistic understanding of muscle physiology and biochemistry compared with typically performed whole-muscle homogenate analyses. This analysis, however, is laborious and typically not p ...[more]
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