Dataset Information

Impact of Hemoglobin S Trait on Cell Surface Antibody Recognition of Plasmodium falciparum-Infected Erythrocytes in Pregnancy-Associated Malaria.

ABSTRACT:

Background

Sickle cell trait (HbAS) confers partial protection against malaria by reducing the adhesion of Plasmodium falciparum-infected erythrocytes to host receptors, but little is known about its potential protection against placental malaria.

Methods

Using flow cytometry, we assessed the recognition of HbAA and HbAS VAR2CSA-expressing infected erythrocytes, by plasma from 159 Beninese pregnant women with either HbAA (normal) or HbAS. Using multivariate linear models adjusted for gravidity, parasite infection at delivery, glucose-6-phosphate dehydrogenase deficiency, and α-thalassemia carriage, we observed significantly reduced cell surface antibody binding of HbAS-infected erythrocytes by plasma from HbAS compared with HbAA women (P < 10^-3).

Results

The difference in cell surface antibody binding was only observed when infected erythrocytes and plasma were associated according to the same hemoglobin genotype. Similar levels of VAR2CSA-specific antibody were measured by enzyme-linked immunosorbent assay in the 2 groups, suggesting that the altered interaction between VAR2CSA and HbAS women's antibodies could reflect abnormal display of VAR2CSA on HbAS erythrocytes.

Conclusions

Our data stress the need for assessments of erythrocyte disorders such as the sickle cell trait in a population group when studying immunological responses to P falciparum.

SUBMITTER: Chauvet M

PROVIDER: S-EPMC6483131 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Publications

Impact of Hemoglobin S Trait on Cell Surface Antibody Recognition of <i>Plasmodium falciparum</i>-Infected Erythrocytes in Pregnancy-Associated Malaria.

Chauvet Margaux M Tétard Marilou M Cottrell Gilles G Aussenac Florentin F Brossier Emeline E Denoyel Luc L Hanny Marion M Lohezic Murielle M Milet Jacqueline J Ndam Nicaise Tuikue NT Pineau Damien D Roman Jocelyne J Luty Adrian J F AJF Gamain Benoît B Migot-Nabias Florence F Merckx Anaïs A

Open forum infectious diseases 20190329 4

<h4>Background</h4>Sickle cell trait (HbAS) confers partial protection against malaria by reducing the adhesion of <i>Plasmodium falciparum</i>-infected erythrocytes to host receptors, but little is known about its potential protection against placental malaria.<h4>Methods</h4>Using flow cytometry, we assessed the recognition of HbAA and HbAS VAR2CSA-expressing infected erythrocytes, by plasma from 159 Beninese pregnant women with either HbAA (normal) or HbAS. Using multivariate linear models ad ...[more]

PMID: 31041352

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