Ontology highlight
ABSTRACT:
SUBMITTER: Chen L
PROVIDER: S-EPMC6484296 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Journal of cellular and molecular medicine 20190320 5
YB1 is a negative regulator in liver fibrosis. We wondered whether SJYB1, a homologous protein of YB1 from Schistosoma japonicum, has an effect on liver fibrosis in vitro. Recombinant SJYB1 (rSJYB1) protein was expressed in a bacterial system and purified by Ni-NTA His·Bind Resin. A human hepatic stellate cell line, the LX-2 cell line, was cultured and treated with rSJYB1. The role of rSJYB1 on LX-2 cells was then analysed by Western blot and luciferase assay. We succeeded in expressing and puri ...[more]