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Glycomimetic affinity-enrichment proteomics identifies partners for a clinically-utilized iminosugar.

ABSTRACT: Widescale evaluation of interacting partners for carbohydrates is an underexploited area. Probing of the 'glyco-interactome' has particular relevance given the lack of direct genetic control of glycoconjugate biosynthesis. Here we design, create and utilize a natural product-derived glycomimetic iminosugar probe in a Glycomimetic Affinity-enrichment Proteomics (glyco-AeP) strategy to elucidate key interactions directly from mammalian tissue. The binding partners discovered here and the associated genomic analysis implicate a subset of chaperone and junctional proteins as important in male fertility. Such repurposing of existing therapeutics thus creates direct routes to probing in vivo function. The success of this strategy suggests a general approach to discovering 'carbohydrate-active' partners in biology.

SUBMITTER: Cruz IN

PROVIDER: S-EPMC6485602 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature

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Glycomimetic affinity-enrichment proteomics identifies partners for a clinically-utilized iminosugar.

Cruz Isa N IN Barry Conor S CS Kramer Holger B HB Chuang C Celeste CC Lloyd Sarah S van der Spoel Aarnoud C AC Platt Frances M FM Yang Min M Davis Benjamin G BG

Chemical science 20130708 9

Widescale evaluation of interacting partners for carbohydrates is an underexploited area. Probing of the 'glyco-interactome' has particular relevance given the lack of direct genetic control of glycoconjugate biosynthesis. Here we design, create and utilize a natural product-derived glycomimetic iminosugar probe in a Glycomimetic Affinity-enrichment Proteomics (glyco-A<sup>e</sup>P) strategy to elucidate key interactions directly from mammalian tissue. The binding partners discovered here and th ...[more]

PMID: 31031905

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