Glycomimetic affinity-enrichment proteomics identifies partners for a clinically-utilized iminosugar.
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ABSTRACT:
SUBMITTER: Cruz IN
PROVIDER: S-EPMC6485602 | biostudies-literature | 2013 Sep
REPOSITORIES: biostudies-literature
Publications
Glycomimetic affinity-enrichment proteomics identifies partners for a clinically-utilized iminosugar.
Cruz Isa N IN Barry Conor S CS Kramer Holger B HB Chuang C Celeste CC Lloyd Sarah S van der Spoel Aarnoud C AC Platt Frances M FM Yang Min M Davis Benjamin G BG
Chemical science 20130708 9
Widescale evaluation of interacting partners for carbohydrates is an underexploited area. Probing of the 'glyco-interactome' has particular relevance given the lack of direct genetic control of glycoconjugate biosynthesis. Here we design, create and utilize a natural product-derived glycomimetic iminosugar probe in a Glycomimetic Affinity-enrichment Proteomics (glyco-A<sup>e</sup>P) strategy to elucidate key interactions directly from mammalian tissue. The binding partners discovered here and th ...[more]
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