Ontology highlight
ABSTRACT:
SUBMITTER: Spenkelink LM
PROVIDER: S-EPMC6486552 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Spenkelink Lisanne M LM Lewis Jacob S JS Jergic Slobodan S Xu Zhi-Qiang ZQ Robinson Andrew A Dixon Nicholas E NE van Oijen Antoine M AM
Nucleic acids research 20190501 8
Single-stranded DNA-binding proteins (SSBs) support DNA replication by protecting single-stranded DNA from nucleolytic attack, preventing intra-strand pairing events and playing many other regulatory roles within the replisome. Recent developments in single-molecule approaches have led to a revised picture of the replisome that is much more complex in how it retains or recycles protein components. Here, we visualize how an in vitro reconstituted Escherichia coli replisome recruits SSB by relying ...[more]