Dataset Information
Tumour Expression of Histone Deacetylases in Uveal Melanoma.
Ontology highlight
ABSTRACT: Purpose
To determine the expression of histone deacetylase enzymes in uveal melanoma tumour cells.Procedures
This is an observational immunohistochemical study of 16 formalin-fixed, paraffin-embedded eyes enucleated for uveal melanoma between January 2001 and March 2002. Haematoxylin and eosin paraffin sections were reviewed for histopathological parameters according to the American Joint Committee on Cancer 7th edition. Sections were then immunohistochemically stained for histone deacetylases 1, 2, 3, 4 and 6 and sirtuin 2 using an automated Leica Bond II platform and Fast Red chromogen, then digitally scanned using Aperio software before assessment of staining.Results
Nuclear expression of histone deacetylases 1, 2, 3, 4 and 6 and of sirtuin 2 was confirmed in uveal melanoma tumour cells. In addition, the tumour cells showed cytoplasmic expression of histone deacetylases 4 and 6 and sirtuin 2. Nuclear and cytoplasmic immunostaining was also seen in intraocular tissues uninvolved by the tumour.Conclusion
Uveal melanoma tumour cells express histone deacetylases 1, 2, 3, 4 and 6 and sirtuin 2, confirming potential tissue targets for histone deacetylase inhibitors.
SUBMITTER: Levinzon L
PROVIDER: S-EPMC6489079 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Publications
Tumour Expression of Histone Deacetylases in Uveal Melanoma.
Ocular oncology and pathology 20180918 3
<h4>Purpose</h4>To determine the expression of histone deacetylase enzymes in uveal melanoma tumour cells.<h4>Procedures</h4>This is an observational immunohistochemical study of 16 formalin-fixed, paraffin-embedded eyes enucleated for uveal melanoma between January 2001 and March 2002. Haematoxylin and eosin paraffin sections were reviewed for histopathological parameters according to the American Joint Committee on Cancer 7th edition. Sections were then immunohistochemically stained for histon ...[more]