Ontology highlight
ABSTRACT: Background
To investigate the effects of the Alisma and Rhizoma decoction on nonalcoholic steatohepatitis (NASH) and to further shed light on the underlying mechanisms of the actions of the Alisma and Rhizoma decoction.Methods
Plasma alanine aminotransferase (ALT) content was determined and liver inflammation and fibrosis were evaluated. Intrahepatocellular malondialdehyde and superoxide dismutase contents were determined using commercially available kits Furthermore, ?-SMA expression in liver tissues was examined by immunohistochemistry and LC3-II was detected by immunoblotting assays.Results
Mice receiving the Alisma and Rhizoma decoction by gastric lavage had significantly lower plasma ALT content and markedly higher hepatic superoxide dismutase activity than mice receiving the methionine-choline deficient (MCD) diet. Furthermore, the decoction aborted MCD-induced increase in liver malondialdehyde content. Immunohistochemistry showed that the decoction suppressed hepatic ?-SMA expression. Our transmission electronic microscopy revealed that the decoction markedly reduced the number of autophagosomes and immunoblotting assays showed that the decoction caused a dose-dependent decrease in LC3-II in hepatic tissues.Conclusion
The Alisma and Rhizoma decoction lessens NASH-associated liver injuries by modulating oxidative stress and autophagy in hepatocytes of mice fed with MCD.
SUBMITTER: Xu L
PROVIDER: S-EPMC6489313 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
Xu Lijun L Jing Menghui M Yang Lijuan L Jin Lei L Gong Peiqiang P Lu Juan J Lin Hui H Wang Jinping J Cao Qin Q Jiang Yuanye Y
BMC complementary and alternative medicine 20190429 1
<h4>Background</h4>To investigate the effects of the Alisma and Rhizoma decoction on nonalcoholic steatohepatitis (NASH) and to further shed light on the underlying mechanisms of the actions of the Alisma and Rhizoma decoction.<h4>Methods</h4>Plasma alanine aminotransferase (ALT) content was determined and liver inflammation and fibrosis were evaluated. Intrahepatocellular malondialdehyde and superoxide dismutase contents were determined using commercially available kits Furthermore, α-SMA expre ...[more]