Autologous hematopoietic stem cell transplantation in systemic sclerosis induces long-lasting changes in B cell homeostasis toward an anti-inflammatory B cell cytokine pattern.

ABSTRACT:

Background

Autologous hematopoietic stem cell transplantation (aHSCT) is performed in patients with aggressive forms of systemic sclerosis (SSc). The profile of B cell reconstitution after aHSCT is not fully understood. The aim of this study was to investigate changes of B cell subsets and cytokine production of B cells in patients with SSc after aHSCT.

Methods

Peripheral blood of six patients with SSc was collected at defined intervals up to 16 months after aHSCT. Immunophenotyping was performed, and B cell function was determined by measuring cytokine secretion in supernatants of stimulated B cell cultures.

Results

Within 1 month after aHSCT, a peak in the percentage of CD38⁺⁺/CD10⁺/IgD⁺ transitional B cells and CD38⁺⁺/CD2

SUBMITTER: Gernert M 

PROVIDER: S-EPMC6489316 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature