Ontology highlight
ABSTRACT:
SUBMITTER: Huang L
PROVIDER: S-EPMC6490959 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Cancer chemotherapy and pharmacology 20190213 5
It is desirable to develop a fast method for quantification of melphalan due to its instability. Here we report a method for quantification of melphalan (MPL) in human plasma using a UPLC-PDA system. Briefly, 50 µL plasma sample was mixed with 25 µL internal standard (2500 ng/mL acetylmelphalan in methanol) and 25 µL 20% trichloroacetic acid, and centrifuged at 21,000 g (15,000 rpm) at 4 °C for 3 min. The supernatant (5 µL) was injected onto an Acquity™ BEH C18 LC column (2.1 × 50 mm, 1.7 µm) an ...[more]