Dataset Information

Rab-mediated trafficking in the secondary cells of Drosophila male accessory glands and its role in fecundity.

ABSTRACT: The male seminal fluid contains factors that affect female post-mating behavior and physiology. In Drosophila, most of these factors are secreted by the two epithelial cell types that make up the male accessory gland: the main and secondary cells. Although secondary cells represent only ~4% of the cells of the accessory gland, their contribution to the male seminal fluid is essential for sustaining the female post-mating response. To better understand the function of the secondary cells, we investigated their molecular organization, particularly with respect to the intracellular membrane transport machinery. We determined that large vacuole-like structures found in the secondary cells are trafficking hubs labeled by Rab6, 7, 11 and 19. Furthermore, these organelles require Rab6 for their formation and many are essential in the process of creating the long-term postmating behavior of females. In order to better serve the intracellular membrane and protein trafficking communities, we have created a searchable, online, open-access imaging resource to display our complete findings regarding Rab localization in the accessory gland.

SUBMITTER: Prince E

PROVIDER: S-EPMC6492190 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Rab-mediated trafficking in the secondary cells of Drosophila male accessory glands and its role in fecundity.

Prince Elodie E Kroeger Benjamin B Gligorov Dragan D Wilson Clive C Eaton Suzanne S Karch François F Brankatschk Marko M Maeda Robert K RK

Traffic (Copenhagen, Denmark) 20181226 2

The male seminal fluid contains factors that affect female post-mating behavior and physiology. In Drosophila, most of these factors are secreted by the two epithelial cell types that make up the male accessory gland: the main and secondary cells. Although secondary cells represent only ~4% of the cells of the accessory gland, their contribution to the male seminal fluid is essential for sustaining the female post-mating response. To better understand the function of the secondary cells, we inve ...[more]

PMID: 30426623

Similar Datasets

Project description:BackgroundHeliconius butterflies possess a remarkable diversity of phenotypes, physiologies, and behaviors that has long distinguished this genus as a focal taxon in ecological and evolutionary research. Recently Heliconius has also emerged as a model system for using genomic methods to investigate the causes and consequences of biological diversity. One notable aspect of Heliconius diversity is a dichotomy in mating systems which provides an unusual opportunity to investigate the relationship between sexual selection and the evolution of reproductive proteins. As a first step in pursuing this research, we report the generation and analysis of expressed sequence tags (ESTs) from the male accessory gland of H. erato and H. melpomene, species representative of the two mating systems present in the genus Heliconius.ResultsWe successfully sequenced 933 ESTs clustering into 371 unigenes from H. erato and 1033 ESTs clustering into 340 unigenes from H. melpomene. Results from the two species were very similar. Approximately a third of the unigenes showed no significant BLAST similarity (E-value <10-5) to sequences in GenBank's non-redundant databases, indicating that a large proportion of novel genes are expressed in Heliconius male accessory glands. In both species only a third of accessory gland unigenes were also found among genes expressed in wing tissue. About 25% of unigenes from both species encoded secreted proteins. This includes three groups of highly abundant unigenes encoding repetitive proteins considered to be candidate seminal fluid proteins; proteins encoded by one of these groups were detected in H. erato spermatophores.ConclusionThis collection of ESTs will serve as the foundation for the future identification and evolutionary analysis of male reproductive proteins in Heliconius butterflies. These data also represent a significant advance in the rapidly growing collection of genomic resources available in Heliconius butterflies. As such, they substantially enhance this taxon as a model system for investigating questions of ecological, phenotypic, and genomic diversity.

Dataset Information

Rab-mediated trafficking in the secondary cells of Drosophila male accessory glands and its role in fecundity.

Publications

Rab-mediated trafficking in the secondary cells of Drosophila male accessory glands and its role in fecundity.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets