An Enzyme-Mediated Amplification Strategy Enables Detection of ?-Lactamase Activity Directly in Unprocessed Clinical Samples for Phenotypic Detection of ?-Lactam Resistance.
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ABSTRACT:
SUBMITTER: deBoer TR
PROVIDER: S-EPMC6494105 | biostudies-literature | 2018 Oct
REPOSITORIES: biostudies-literature
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An Enzyme-Mediated Amplification Strategy Enables Detection of β-Lactamase Activity Directly in Unprocessed Clinical Samples for Phenotypic Detection of β-Lactam Resistance.
deBoer Tara R TR Tarlton Nicole J NJ Yamaji Reina R Adams-Sapper Sheila S Wu Tiffany Z TZ Maity Santanu S Vesgesna Giri K GK Sadlowski Corinne M CM DePaola Peter P Riley Lee W LW Murthy Niren N
Chembiochem : a European journal of chemical biology 20180926 20
Biochemical assays that can identify β-lactamase activity directly from patient samples have the potential to significantly improve the treatment of bacterial infections. However, current β-lactamase probes do not have the sensitivity needed to measure β-lactam resistance directly from patient samples. Here, we report the development of an instrument-free signal amplification technology, DETECT, that connects the activity of two enzymes in series to effectively amplify the activity of β-lactamas ...[more]
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