Dataset Information

Poly(?-caprolactone)-based substrates bearing pendant small chemical groups as a platform for systemic investigation of chondrogenesis.

ABSTRACT:

Objectives

Physiochemical properties of biomaterials play critical roles in dictating types of cell behaviour. In this study, a series of poly(?-caprolactone) (PCL)-derived polymers bearing different small chemical groups was employed as a platform to evaluate chondrogenesis of different cell types.

Materials and methods

Thin films were prepared by spin-coating PCL derivatives. Rabbit articular chondrocytes (rACs) and rabbit bone marrow-derived mesenchymal stem cells (rMSCs) were seeded on to the films, and cell adhesion, proliferation, extracellular matrix production and gene expression were evaluated.

Results

The presence of hydrophilic groups (-NH2 , -COOH, -OH and -C=O) promoted adhesion and proliferation of primary rACs and rMSCs. On these polymeric films, chondrogenesis of primary rACs depended on culture time. For passaged cells, re-differentiation was induced on these films by chondrogenic induction, but less for cells of passage 5 compared to passage 3. While films with hydrophilic groups favoured chondrocytic gene expression of both types of passaged cells, production of glycosaminoglycans (GAG) was similar for those of passage 3 on all films, and PCL-CH3 film better supported GAG production for cells of passage 5. Under chondrogenic conditions, rMSCs were more efficient at GAG production on PCL and PCL-NH2 films.

Conclusions

This study demonstrates that different cells displayed distinct responses to substrate surface chemistry, implying that cell-biomaterial interactions can be developmental stage dependent. This provides a novel perspective for developing biomaterials for cartilage regeneration.

SUBMITTER: Chen M

PROVIDER: S-EPMC6495506 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature

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Publications

Poly(ε-caprolactone)-based substrates bearing pendant small chemical groups as a platform for systemic investigation of chondrogenesis.

Chen Min M Xu Lei L Zhou Yan Y Zhang Yan Y Lang Meidong M Ye Zhaoyang Z Tan Wen-Song WS

Cell proliferation 20160630 4

<h4>Objectives</h4>Physiochemical properties of biomaterials play critical roles in dictating types of cell behaviour. In this study, a series of poly(ε-caprolactone) (PCL)-derived polymers bearing different small chemical groups was employed as a platform to evaluate chondrogenesis of different cell types.<h4>Materials and methods</h4>Thin films were prepared by spin-coating PCL derivatives. Rabbit articular chondrocytes (rACs) and rabbit bone marrow-derived mesenchymal stem cells (rMSCs) were ...[more]

PMID: 27364032

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