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MiR-142-3p inhibits cancer cell proliferation by targeting CDC25C.

ABSTRACT:

Objectives

MicroRNAs (miRNAs) contribute to control of cell cycle progression and are frequently deregulated in cancer. The focus of this study was to determine effects of miR-142-3p on the cell cycle progression and cancer cell proliferation.

Materials and methods

RT-qPCR was performed to determine expression of miR-142-3p in a range of cancer cell lines and in clinical cancer specimens. To further understand its role, we restored its expression in cancer cell lines by transfection with miR-142-3p mimics or inhibitors. Effects of miR-142-3p on cell cycle progression and cell proliferation were also determined.

Results

miR-142-3p was down-regulated in both cancer cell lines and cancer specimens. Its overexpression suppressed proliferation, whereas its depletion promoted it. In addition, miR-142-3p lead to cell cycle arrest in G2/M. Moreover, CDC25C was identified as being a target of miR-142-3p, ectopic expression of which reversed suppression of cell proliferation.

Conclusions

Our observations suggest that miR-142-3p functioned as a tumor suppressor by targeting CDC25C.

SUBMITTER: Cao XC

PROVIDER: S-EPMC6496930 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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miR-142-3p inhibits cancer cell proliferation by targeting CDC25C.

Cao Xu-Chen XC Yu Yue Y Hou Li-Kun LK Sun Xiao-Hu XH Ge Jie J Zhang Bin B Wang Xin X

Cell proliferation 20160125 1

<h4>Objectives</h4>MicroRNAs (miRNAs) contribute to control of cell cycle progression and are frequently deregulated in cancer. The focus of this study was to determine effects of miR-142-3p on the cell cycle progression and cancer cell proliferation.<h4>Materials and methods</h4>RT-qPCR was performed to determine expression of miR-142-3p in a range of cancer cell lines and in clinical cancer specimens. To further understand its role, we restored its expression in cancer cell lines by transfecti ...[more]

PMID: 26805039

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