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Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex.

ABSTRACT: Ebola virus is an emerging virus that is capable of causing a deadly disease in humans. Replication, transcription and packaging of the viral genome are carried out by the viral nucleocapsid. The nucleocapsid is a complex of the viral nucleoprotein, RNA and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins. The 3.1?Å resolution single-particle cryo-electron microscopy structure of the nucleoprotein-RNA helical filament presented here resembles previous structures determined at lower resolution, while providing improved molecular details of protein-protein and protein-RNA interactions. The higher resolution of the structure presented here will facilitate the design and characterization of novel and specific Ebola virus therapeutics targeting the nucleocapsid.

SUBMITTER: Kirchdoerfer RN

PROVIDER: S-EPMC6497097 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Cryo-EM structure of the Ebola virus nucleoprotein-RNA complex.

Kirchdoerfer Robert N RN Saphire Erica Ollmann EO Ward Andrew B AB

Acta crystallographica. Section F, Structural biology communications 20190424 Pt 5

Ebola virus is an emerging virus that is capable of causing a deadly disease in humans. Replication, transcription and packaging of the viral genome are carried out by the viral nucleocapsid. The nucleocapsid is a complex of the viral nucleoprotein, RNA and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins. The 3.1 Å resolution single-particle cryo-electron microscopy structure of the nucleoprotein-RNA h ...[more]

PMID: 31045563

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