Browse
Submit Data
Databases
API
Help

Dataset Information

25 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome Sequencing of Immature Ants Reveals the Complete Genome Sequence of a New Isolate of Solenopsis invicta Virus 2 from the Mississippi Delta.

ABSTRACT: Solenopsis invicta virus 2 (SINV-2) is an RNA virus that infects red imported fire ants. I report the genome sequence of SINV-2MSD, an isolate infecting ants collected from Mississippi. The obtained genome is 11,303 nucleotides, including six open reading frames encoding four structural proteins, a helicase, and an RNA-dependent RNA polymerase.

SUBMITTER: Allen ML

PROVIDER: S-EPMC6498243 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Transcriptome Sequencing of Immature Ants Reveals the Complete Genome Sequence of a New Isolate of Solenopsis invicta Virus 2 from the Mississippi Delta.

Allen Margaret L ML

Microbiology resource announcements 20190502 18

Solenopsis invicta virus 2 (SINV-2) is an RNA virus that infects red imported fire ants. I report the genome sequence of SINV-2MSD, an isolate infecting ants collected from Mississippi. The obtained genome is 11,303 nucleotides, including six open reading frames encoding four structural proteins, a helicase, and an RNA-dependent RNA polymerase. ...[more]

PMID: 31048388

Similar Datasets

Complete Genome Sequence of a New Isolate of Solenopsis invicta virus 3 from Solenopsis invicta × richteri Hybrid Ants.

Project description:Solenopsis invicta virus 3 (SINV-3) is a positive-sense, single-stranded RNA virus that infects the red imported fire ant, Solenopsis invicta Buren. We report here the full genome (10,383 nucleotides) of an isolate infecting Solenopsis invicta × richteri hybrid ants, which we have identified as SINV-3 hybrid.

| S-EPMC5722059 | biostudies-literature

Metabolic scaling of fire ants (Solenopsis invicta) engaged in collective behaviors.

Project description:During flash floods, fire ants (Solenopsis invicta Buren) link their bodies together to build rafts to stay afloat, and towers to anchor onto floating vegetation. Can such challenging conditions facilitate synchronization and coordination, resulting in energy savings per capita? To understand how stress affects metabolic rate, we used constant-volume respirometry to measure the metabolism of fire ant workers. Group metabolic rates were measured in a series of conditions: at normal state, at three elevated temperatures, during rafting, and during tower-building. We hypothesized that the metabolic rate of ants at various temperatures would scale isometrically (proportionally with the group mass). Indeed, we found metabolic rates scaled isometrically under all temperature conditions, giving evidence that groups of ants differ from entire colonies, which scale allometrically. We then hypothesized that the metabolism of ants engaged in rafting and tower-building would scale allometrically. We found partial evidence for this hypothesis: ants rafting for short times had allometric metabolic rates, but this effect vanished after 30 min. Rafting for long times and tower-building both scaled isometrically. Tower-building consumed the same energy per capita as ants in their normal state. Rafting ants consumed almost 43% more energy than ants in their normal state, with smaller rafts consuming more energy per capita. Together, our results suggest that stressful conditions requiring coordination can influence metabolic demand. This article has an associated First Person interview with the first author of the paper.

| S-EPMC8905630 | biostudies-literature

Unique venom proteins from Solenopsis invicta x Solenopsis richteri hybrid fire ants.

Project description:The Solenopsis venom protein 2 transcript was amplified, sequenced, probed, and analyzed from Solenopsis invicta x Solenopsis richteri hybrid ant colonies (hybrids) collected from across Tennessee to determine the extent of introgression of each parent allele (Solenopsis invicta venom protein 2 [Soli2] and Solenopsis richteri venom protein 2 [Solr2]). Chemotaxonomic analyses of venom alkaloids and cuticular hydrocarbons were used to categorize hybrid colonies and their relative relatedness to each parent species. Hybrid colonies were chosen randomly from each chemotaxonomic hybridization category, including "very near S. richteri," "near S. richteri," "near S. invicta," and "very near S. invicta." Lateral flow immunoassays for detection of the Soli2 and Solr2 venom proteins were largely in agreement with the chemotaxonomic analyses for the very near S. richteri (100% Solr2) and very near S. invicta (80% Soli2, 20% Soli2 + Solr2 detected in the sample) groups, while Soli2 and Solr2 were reported in 60% and 40% in the near S. invicta and near S. richteri chemotaxonomic groups. Analysis of transcripts from the hybrid colonies revealed a sequence with 100% identity to Soli2 (GenBank Accession L09560) and three unique sequences, which we identify as Solenopsis hybrid venom protein 2 (Solh2; GenBank Accession MT150127), Solenopsis hybrid truncated venom protein 2 (Solh2Tr97; Genbank Accession MT150129), and Solenopsis richteri venom protein 2, D to A change at position 69 (Solr2A69; GenBank Accession MT150128). The predicted open reading frame for Solh2 and Solh2Tr97 revealed sequences unique to hybrid ants, with Solh2Tr97an alternatively spliced form. A third unique sequence, Solr2A69, is likely the correct sequence for Solr2, which appears to have been published previously with a sequencing error (GenBank Accession P35776).

| S-EPMC8131721 | biostudies-literature

Insecticidal and Enzyme Inhibitory Activities of Isothiocyanates against Red Imported Fire Ants, Solenopsis invicta.

Project description:Contact and fumigation toxicity of four isothiocyanates (ITCs), including allyl isothiocyanate (AITC), 3-butenyl isothiocyanate (3BITC), 3-(methylthio) propyl isothiocyanate (3MPITC) and 2-phenylethyl isothiocyanate (2PEITC), were evaluated against the red imported fire ant worker, Solenopsis invicta Buren. 2PEITC and 3MPITC exhibited strong contact toxicity. The median lethal dose (LD50)value of AITC, 2PEITC and 3MPITC were 7.99, 2.36 and 2.09 µg/ant respectively. In addition, AITC and 3MPITC also showed strong fumigation toxicity but not 2PEITC. The median lethal concentration (LC50) values of AITC and 3MPITC were 32.49 and 57.6 µg/L, respectively. In contrast, 3BITC did not exhibit any contact and fumigation toxicity even at 100 ?g/?L. Esterase (EST), glutathione S-transferase (GST) and acetylcholinesterase (AChE)-inhibiting activities were assessed for three ITCs in S. invicta workers. All three ITCs inhibited both EST and GST activities but not AChE. The in vitro half maximal inhibitory concentration (IC50)values of AITC, 2PEITC and 3MPITC for GST were 3.32, 0.61 and 0.66 µg/µL, respectively. These results suggested that naturally occurring ITCs might be potentially useful for developing fire ants control products.

| S-EPMC7277602 | biostudies-literature

Impact of imidacloprid on new queens of imported fire ants, Solenopsis invicta (Hymenoptera: Formicidae).

Project description:Neonicotinoid insecticides are commonly used in managing pest insects, including the imported fire ant, Solenopsis invicta Buren. There is increasing evidence that neonicotinoid insecticides at sublethal concentrations have profound effects on social insects. However, the sublethal effect of neonicotinoids on S. invicta has never been investigated. In this study, the newly mated queens were fed with water containing 0.01 or 0.25 ?g/ml imidacloprid. Imidacloprid at both concentrations did not cause any increase in queen mortality during the founding stage; however, it significantly reduced queens' brood tending ability. In the 0.25 ?g/ml imidacloprid treatment, the time to larval emergence was significantly delayed and no pupae or adult workers were produced. This study provides clear evidence that imidacloprid at sublethal concentrations has a significant detrimental impact on S. invicta queens and the development of incipient colonies.

| S-EPMC4672302 | biostudies-literature

Esterase in imported fire ants, Solenopsis invicta and S. richteri (Hymenoptera: Formicidae): activity, kinetics and variation.

Project description:Solenopsis invicta and Solenopsis richteri are two closely related invasive ants native to South America. Despite their similarity in biology and behavior, S. invicta is a more successful invasive species. Toxic tolerance has been found to be important to the success of some invasive species. Esterases play a crucial role in toxic tolerance of insects. Hence, we hypothesized that the more invasive S. invicta would have a higher esterase activity than S. richteri. Esterase activities were measured for workers and male and female alates of both ant species using α-naphthyl acetate and β-naphthyl acetate as substrates. Esterase activities in S. invicta were always significantly higher than those in S. richteri supporting our hypothesis. In S. invicta, male alates had the highest esterase activities followed by workers then female alates for both substrates. In S. richetri, for α-naphthyl acetate, male alates had the highest activity followed by female alates then workers, while for β-naphthyl acetate, female alates had the highest activity followed by male alates then workers. For workers, S. richteri showed significantly higher levels of variation about the mean esterase activity than S. invicta. However, S. invicta showed significantly higher levels of variation in both female and male alates.

| S-EPMC5382690 | biostudies-literature

Paving Behavior in Ants and Its Potential Application in Monitoring Two Urban Pest Ants, Solenopsis invicta and Tapinoma melanocephalum.

Project description:Our previous study discovered that two urban pest ants, red imported fire ants, Solenopsis invicta Buren (Formicidae: Myrmicinae), and ghost ants, Tapinoma melanocephalum (Fabricius) (Formicidae: Dolichoderinae), can pave viscose surfaces with particles to facilitate food search and transport. We hypothesize that this paving behavior can be applied to monitor S. invicta and T. melanocephalum. In the present study, 3998 adhesive tapes, each with a food source (sausage), were placed in 20 locations around Guangzhou, China (181-224 tapes per location), and their efficiency to detect S. invicta and T. melanocephalum was compared with two traditional ant-monitoring methods, baiting and pitfall trapping. Overall, S. invicta was detected by 45.6% and 46.4% of baits and adhesive tapes, respectively. In each location, the percentage of adhesive tapes detecting S. invicta and T. melanocephalum was similar when compared to baits and pitfall traps. However, significantly more non-target ant species showed up on bait and pitfall traps. Seven non-target ant species-Pheidole parva Mayr (Formicidae: Myrmicinae), Pheidole nodus Smith (Formicidae: Myrmicinae), Pheidole sinica Wu & Wang (Formicidae: Myrmicinae), Pheidole yeensis Forel (Formicidae: Myrmicinae), Carebara affinis (Jerdon) (Formicidae: Myrmicinae), Camponotus nicobarensis Mayr (Formicidae: Formicinae), and Odontoponera transversa (Smith) (Formicidae: Ponerinae)-also showed tape paving behavior, but they can be easily distinguished morphologically from S. invicta and T. melanocephalum. Our study showed that the paving behavior occurs in different subfamilies of ants (i.e., myrmicinae, dolichoderinae, formicinae, and ponerinae). In addition, paving behavior can potentially be used to develop more specific monitoring methods for S. invicta and T. melanocephalum in urban areas in southern China.

| S-EPMC10054563 | biostudies-literature

Decreased small mammal and on-host tick abundance in association with invasive red imported fire ants (Solenopsis invicta).

Project description:Invasive species may impact pathogen transmission by altering the distributions and interactions among native vertebrate reservoir hosts and arthropod vectors. Here, we examined the direct and indirect effects of the red imported fire ant (Solenopsis invicta) on the native tick, small mammal and pathogen community in southeast Texas. Using a replicated large-scale field manipulation study, we show that small mammals were more abundant on treatment plots where S. invicta populations were experimentally reduced. Our analysis of ticks on small mammal hosts demonstrated a threefold increase in the ticks caught per unit effort on treatment relative to control plots, and elevated tick loads (a 27-fold increase) on one common rodent species. We detected only one known human pathogen (Rickettsia parkeri), present in 1.4% of larvae and 6.7% of nymph on-host Amblyomma maculatum samples but with no significant difference between treatment and control plots. Given that host and vector population dynamics are key drivers of pathogen transmission, the reduced small mammal and tick abundance associated with S. invicta may alter pathogen transmission dynamics over broader spatial scales.

| S-EPMC5046925 | biostudies-literature

A combined protein toxin screening based on the transcriptome and proteome of Solenopsis invicta.

Project description:BackgroundMulti-omics technology provides a good tool to analyze the protein toxin composition and search for the potential pathogenic factors of Solenopsis invicta, under the great harm of the accelerated invasion in southern China.MethodsSpecies collection, functional annotation, toxin screening, and 3D modeling construction of three interested toxins were performed based on the successfully constructed transcriptome and proteome of S. invicta.ResultsA total of 33,231 unigenes and 721 proteins were obtained from the constructed transcriptome and proteome, of which 9,842 (29.62%) and 4,844 (14.58%) unigenes, as well as 469 (65.05%) and 71 (99.45%) proteins were annotated against the databases of Gene Ontology and Kyoto Encyclopedia of Genes and Genomes, respectively. After comparing with the uniprot toxin database, a total of 316 unigenes and 47 proteins (calglandulin, venom allergen 3, and venom prothrombin activator hopsarin-D, etc.) were successfully screened.ConclusionsThe update of annotations at the transcriptome and proteome levels presents a progression in the comprehension of S. invicta in China. We also provide a protein toxin list that could be used for further exploration of toxicity as well as its antagonistic strategy by S. invicta.

| S-EPMC9494847 | biostudies-literature

Near-Complete Genome Sequences of New Strains of Nylanderia Fulva Virus 1 from Solenopsis invicta.

Project description:Nylanderia fulva virus 1 (NfV-1) is a single-stranded positive-sense RNA virus that infects the tawny crazy ant. Three near-complete genomes of NfV-1SI (92% to 94% nucleotide identity to reference strain NfV-1) found infecting the red imported fire ant were determined. The genomes have 10,904 to 10,918 nucleotides and include most of the coding region for the polyprotein.

| S-EPMC7380534 | biostudies-literature