Rapid detection of invasive Mycobacterium chimaera disease via a novel plasma-based next-generation sequencing test.

ABSTRACT:

Background

There is an ongoing outbreak of Mycobacterium chimaera infections among patients exposed to contaminated heater-cooler devices used during cardiac surgery. Recognition of M. chimaera infection is hampered by its long latency and non-specific symptoms. Standard diagnostic methods using acid-fast bacilli (AFB) culture often require invasive sampling, have low sensitivity, and can take weeks to result. We describe the performance of a plasma-based next-generation sequencing test (plasma NGS) for the diagnosis of M. chimaera infection.

Methods

We conducted a retrospective study of 10 patients with a history of cardiac surgery who developed invasive M. chimaera infection and underwent testing by plasma NGS between February 2017 and April 2018.

Results

Plasma NGS

SUBMITTER: Nomura J 

PROVIDER: S-EPMC6498503 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature