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ABSTRACT: Background
The protective efficacy of the most promising malaria whole-parasite based vaccine candidates critically depends on the parasite's potential to migrate in the human host. Key components of the parasite motility machinery (e.g. adhesive proteins, actin/myosin-based motor, geometrical properties) have been identified, however the regulation of this machinery is an unknown process.Methods
In vitro microscopic live imaging of parasites in different formulations was performed and analysed, with the quantitative analysis software SMOOTIn vitro, their motility; their adherence capacity, movement pattern and velocity during forward locomotion.Results
SMOOTIn vitro enabled the detailed analysis of the regulation of the motility machinery of Plasmodium berghei in response to specific (macro)molecules in the formulation. Albumin acted as an essential supplement to induce parasite attachment and movement. Glucose, salts and other whole serum components further increased the attachment rate and regulated the velocity of the movement.Conclusions
Based on the findings can be concluded that a complex interplay of albumin, glucose and certain salts and amino acids regulates parasite motility. Insights in parasite motility regulation by supplements in solution potentially provide a way to optimize the whole-parasite malaria vaccine formulation.
SUBMITTER: de Korne CM
PROVIDER: S-EPMC6498664 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
de Korne Clarize M CM Lageschaar Luuk T LT van Oosterom Matthias N MN Baalbergen Els E Winkel Beatrice M F BMF Chevalley-Maurel Severine C SC Velders Aldrik H AH Franke-Fayard Blandine M D BMD van Leeuwen Fijs W B FWB Roestenberg Meta M
Malaria journal 20190502 1
<h4>Background</h4>The protective efficacy of the most promising malaria whole-parasite based vaccine candidates critically depends on the parasite's potential to migrate in the human host. Key components of the parasite motility machinery (e.g. adhesive proteins, actin/myosin-based motor, geometrical properties) have been identified, however the regulation of this machinery is an unknown process.<h4>Methods</h4>In vitro microscopic live imaging of parasites in different formulations was perform ...[more]