Protein Kinase C ? Regulates the Depletion of Actin at the Immunological Synapse Required for Polarized Exosome Secretion by T Cells.

ABSTRACT: Multivesicular bodies (MVB) are endocytic compartments that enclose intraluminal vesicles (ILVs) formed by inward budding from the limiting membrane of endosomes. In T lymphocytes, ILVs are secreted as Fas ligand-bearing, pro-apoptotic exosomes following T cell receptor (TCR)-induced fusion of MVB with the plasma membrane at the immune synapse (IS). In this study we show that protein kinase C ? (PKC?), a novel PKC isotype activated by diacylglycerol (DAG), regulates TCR-controlled MVB polarization toward the IS and exosome secretion. Concomitantly, we demonstrate that PKC?-interfered T lymphocytes are defective in activation-induced cell death. Using a DAG sensor based on the C1 DAG-binding domain of PKC? and a GFP-PKC? chimera, we reveal that T lymphocyte activation enhances DAG levels at the MVB endomembranes which mediates the association of PKC? to MVB. Spatiotemporal reorganization of F-actin at the IS is inhibited in PKC?-interfered T lymphocytes. Therefore, we propose PKC? as a DAG effector that regulates the actin reorganization necessary for MVB traffic and exosome secretion.

SUBMITTER: Herranz G 

PROVIDER: S-EPMC6499072 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature