Dataset Information

Overexpression of the ChVDE gene, encoding a violaxanthin de-epoxidase, improves tolerance to drought and salt stress in transgenic Arabidopsis.

ABSTRACT: To investigate the protective mechanism of violaxanthin de-epoxidase (VDE) zeaxanthin in Cerasus humilis under drought and salt-stress conditions, we cloned the entire cDNA sequence of ChVDE from C. humilis and generated ChVDE-overexpression (OE) and ChVDE-complementation (CE) Arabidopsis plants. The open reading frame of ChVDE contained 1,446 bp nucleotides and encoded 481 amino acids. The ChVDE showed the highest similarity with those of Camellia sinensis and Citrus sinensis. Subcellular localization analysis showed that ChVDE was located in the chloroplasts. OE plants showed stronger root growth and higher levels of total chlorophyll as compared to WT and VDE mutant (npq1-2) plants. Moreover, the relative de-epoxidation state of the xanthophyll cycle pigments (A?+?Z)/(V?+?A+Z) was higher in OE plants than in the controls. OE plants had enhanced photosynthetic rates, respiration rates, and transpiration rates compared with the WT or npq1-2 plants after drought or salt treatment. Collectively, our results demonstrate that ChVDE plays a positive role in both drought and salt tolerance.

SUBMITTER: Sun LN

PROVIDER: S-EPMC6499860 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Overexpression of the ChVDE gene, encoding a violaxanthin de-epoxidase, improves tolerance to drought and salt stress in transgenic Arabidopsis.

Sun Li Na LN Wang Fang F Wang Jie Wan JW Sun Li Jiao LJ Gao Wen Rui WR Song Xing Shun XS

3 Biotech 20190503 5

To investigate the protective mechanism of violaxanthin de-epoxidase (VDE) zeaxanthin in Cerasus humilis under drought and salt-stress conditions, we cloned the entire cDNA sequence of ChVDE from C. humilis and generated ChVDE-overexpression (OE) and ChVDE-complementation (CE) Arabidopsis plants. The open reading frame of ChVDE contained 1,446 bp nucleotides and encoded 481 amino acids. The ChVDE showed the highest similarity with those of Camellia si ...[more]

PMID: 31065497

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Dataset Information

Overexpression of the ChVDE gene, encoding a violaxanthin de-epoxidase, improves tolerance to drought and salt stress in transgenic Arabidopsis.

Publications

Overexpression of the <i>ChVDE</i> gene, encoding a violaxanthin de-epoxidase, improves tolerance to drought and salt stress in transgenic Arabidopsis.

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