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Altered Cervical Mucosal Gene Expression and Lower Interleukin 15 Levels in Women With Schistosoma haematobium Infection but Not in Women With Schistosoma mansoni Infection.


ABSTRACT:

Background

Schistosomiasis increases the risk of human immunodeficiency virus (HIV) acquisition in women by mechanisms that are incompletely defined. Our objective was to determine how the cervical environment is impacted by Schistosoma haematobium or Schistosoma mansoni infection by quantifying gene expression in the cervical mucosa and cytokine levels in cervicovaginal lavage fluid.

Methods

We recruited women with and those without S. haematobium infection and women with and those without S. mansoni infection from separate villages in rural Tanzania with high prevalences of S. haematobium and S. mansoni, respectively. Infection status was determined by urine and stool microscopy and testing for serum circulating anodic antigen. RNA was extracted from cervical cytobrush samples for transcriptome analysis. Cytokine levels were measured by magnetic bead immunoassay.

Results

In the village where S. haematobium was prevalent, 110 genes were differentially expressed in the cervical mucosa of 18 women with versus 39 without S. haematobium infection. Among the 27 cytokines analyzed in cervicovaginal lavage fluid from women in this village, the level of interleukin 15 was lower in the S. haematobium-infected group (62.8 vs 102.9 pg/mL; adjusted P = .0013). Differences were not observed in the S. mansoni-prevalent villages between 11 women with and 29 without S. mansoni infection.

Conclusions

We demonstrate altered cervical mucosal gene expression and lower interleukin 15 levels in women with S. haematobium infection as compared to those with S. mansoni infection, which may influence HIV acquisition and cancer risks. Studies to determine the effects of antischistosome treatment on these mucosal alterations are needed.

SUBMITTER: Dupnik KM 

PROVIDER: S-EPMC6500550 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Altered Cervical Mucosal Gene Expression and Lower Interleukin 15 Levels in Women With Schistosoma haematobium Infection but Not in Women With Schistosoma mansoni Infection.

Dupnik Kathryn M KM   Lee Myung Hee MH   Mishra Pallavi P   Reust Mary Juliet MJ   Colombe Soledad S   Haider Syeda Razia SR   Yao Benjamin B   Vick Kaitlin K   Zhang Tuo T   Xiang Jenny J   Miyaye Donald D   Magawa Ruth R   Lyimo Eric E   Mukerebe Crispin C   Mngara Julius J   Kalluvya Samuel E SE   de Dood Claudia J CJ   van Dam Govert J GJ   Corstjens Paul L A M PLAM   Downs Jennifer A JA  

The Journal of infectious diseases 20190501 11


<h4>Background</h4>Schistosomiasis increases the risk of human immunodeficiency virus (HIV) acquisition in women by mechanisms that are incompletely defined. Our objective was to determine how the cervical environment is impacted by Schistosoma haematobium or Schistosoma mansoni infection by quantifying gene expression in the cervical mucosa and cytokine levels in cervicovaginal lavage fluid.<h4>Methods</h4>We recruited women with and those without S. haematobium infection and women with and tho  ...[more]

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