Blood Microbiome Profile in CKD : A Pilot Study.

ABSTRACT:

Background and objectives

The association between gut dysbiosis, high intestinal permeability, and endotoxemia-mediated inflammation is well established in CKD. However, changes in the circulating microbiome in patients with CKD have not been studied. In this pilot study, we compare the blood microbiome profile between patients with CKD and healthy controls using 16S ribosomal DNA sequencing.

Design, setting, participants, & measurements

Blood bacterial DNA was studied in buffy coat samples quantitatively by 16S PCR and qualitatively by 16S targeted metagenomic sequencing using a molecular pipeline specifically optimized for blood samples in a cross-sectional study comparing 20 nondiabetic patients with CKD and 20 healthy controls.

Results

There were 22 operational ta

SUBMITTER: Shah NB 

PROVIDER: S-EPMC6500932 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature