Characterization of genomic DNA of lactic acid bacteria for activation of plasmacytoid dendritic cells.

ABSTRACT:

Background

Lactococcus lactis strain Plasma (LC-Plasma) possesses strong stimulatory activity for plasmacytoid dendritic cells (pDCs) via the TLR9-Myd88 pathway. To reveal the effective lactic acid bacteria (LAB) genome structure for pDCs stimulatory activity, we performed in vitro screening, using randomly selected 200?bp DNA fragments from the LC-Plasma genome.

Results

We found that the CpG motif copy number in the fragments was positively and significantly correlated with pDCs stimulatory activity (R?=?0.491, p?2) was 0.24, which means other factors affecte activity. We found that the G?+?C contents of the fragment showed a significant negative correlation with activity (R?=?-?0.474, p?ConclusionsOur data suggested that the G?+?C content of DNA is one of the critical factors for pDCs stimulatory activity by DNA fragments. Furthermore, we found that the copy number in the low-G?+?C regions strongly affected the pDCs stimulatory activity of whole cells of LAB strains. These results should be useful for the design of new DNA fragments containing CpG motifs. This study also demonstrated an in silico screening method for identifying bacterial species that are able to activate pDCs.

SUBMITTER: Horie A 

PROVIDER: S-EPMC6501324 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature