Ontology highlight
ABSTRACT:
SUBMITTER: Jones W
PROVIDER: S-EPMC6502812 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Jones Wendell W Greytak Sarah S Odeh Hana H Guan Ping P Powers Jason J Bavarva Jasmin J Moore Helen M HM
Scientific reports 20190506 1
The National Cancer Institute conducted the Biospecimen Pre-analytical Variables (BPV) study to determine the effects of formalin fixation and delay to fixation (DTF) on the analysis of nucleic acids. By performing whole transcriptome sequencing and small RNA profiling on matched snap-frozen and FFPE specimens exposed to different delays to fixation, this study aimed to determine acceptable delays to fixation and proper workflow for accurate and reliable Next-Generation Sequencing (NGS) analysis ...[more]