Unknown

Dataset Information

0

Whole-genome sequencing reveals nosocomial Clostridioides difficile transmission and a previously unsuspected epidemic scenario.


ABSTRACT: To trace the routes and frequencies of transmission of Clostridioides difficile in a tertiary-care hospital in Madrid (Spain), we sequenced the genomes from all C. difficile isolates collected over 36 months (2014-2016) that were indistinguishable from any other isolate by PCR ribotyping. From a total of 589 C. difficile infection cases, we cultivated and PCR-ribotyped 367 C. difficile isolates (62%), of which 265 were genome-sequenced. Based on close relatedness of successively collected isolates (?2 SNPs difference in their genomes), whole-genome sequencing revealed a total of 17 independent, putative transmission clusters, caused by various C. difficile strains and each containing 2 to 18 cases, none of which had been detected previously by standard epidemiological surveillance. Proportions of linked isolates varied widely among PCR ribotypes, from 3% (1/36) for ribotype 014/020 to 60% (12/20) for ribotype 027, suggesting differential aptitudes for nosocomial spread. Remarkably, only a minority (17%) of transmission recipients had direct ward contact to their presumed donors and specific C. difficile genome types frequently went undetectable for several months before re-emerging later, suggesting reservoirs for the pathogen outside of symptomatic patients. Taken together, our analysis based on genome sequencing suggested considerable within-hospital epidemic spread of C. difficile, even though epidemiological data initially had been inconspicuous.

SUBMITTER: Garcia-Fernandez S 

PROVIDER: S-EPMC6502822 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

Whole-genome sequencing reveals nosocomial Clostridioides difficile transmission and a previously unsuspected epidemic scenario.

García-Fernández Sergio S   Frentrup Martinique M   Steglich Matthias M   Gonzaga Aitor A   Cobo Marta M   López-Fresneña Nieves N   Cobo Javier J   Morosini María-Isabel MI   Cantón Rafael R   Del Campo Rosa R   Nübel Ulrich U  

Scientific reports 20190506 1


To trace the routes and frequencies of transmission of Clostridioides difficile in a tertiary-care hospital in Madrid (Spain), we sequenced the genomes from all C. difficile isolates collected over 36 months (2014-2016) that were indistinguishable from any other isolate by PCR ribotyping. From a total of 589 C. difficile infection cases, we cultivated and PCR-ribotyped 367 C. difficile isolates (62%), of which 265 were genome-sequenced. Based on close relatedness of successively collected isolat  ...[more]

Similar Datasets

| PRJEB28391 | ENA
| S-EPMC8754133 | biostudies-literature
| S-EPMC7751860 | biostudies-literature
| S-EPMC4942892 | biostudies-literature
| S-EPMC5575120 | biostudies-literature
| S-EPMC6423542 | biostudies-literature
| S-EPMC6711918 | biostudies-literature
| S-EPMC8204696 | biostudies-literature
| S-EPMC8386809 | biostudies-literature