Dataset Information

Haem iron reshapes colonic luminal environment: impact on mucosal homeostasis and microbiome through aldehyde formation.

ABSTRACT:

Background

The World Health Organization classified processed and red meat consumption as "carcinogenic" and "probably carcinogenic", respectively, to humans. Haem iron from meat plays a role in the promotion of colorectal cancer in rodent models, in association with enhanced luminal lipoperoxidation and subsequent formation of aldehydes. Here, we investigated the short-term effects of this haem-induced lipoperoxidation on mucosal and luminal gut homeostasis including microbiome in F344 male rats fed with a haem-enriched diet (1.5??mol/g) 14-21?days.

Results

Changes in permeability, inflammation, and genotoxicity observed in the mucosal colonic barrier correlated with luminal haem and lipoperoxidation markers. Trapping of luminal haem-induced aldehydes normalised cellular genotoxicity, permeability, and ROS formation on a colon epithelial cell line. Addition of calcium carbonate (2%) to the haem-enriched diet allowed the luminal haem to be trapped in vivo and counteracted these haem-induced physiological traits. Similar covariations of faecal metabolites and bacterial taxa according to haem-induced lipoperoxidation were identified.

Conclusions

This integrated approach provides an overview of haem-induced modulations of the main actors in the colonic barrier. All alterations were closely linked to haem-induced lipoperoxidation, which is associated with red meat-induced colorectal cancer risk.

SUBMITTER: Martin OCB

PROVIDER: S-EPMC6503375 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Publications

Haem iron reshapes colonic luminal environment: impact on mucosal homeostasis and microbiome through aldehyde formation.

Martin Océane C B OCB Olier Maïwenn M Ellero-Simatos Sandrine S Naud Nathalie N Dupuy Jacques J Huc Laurence L Taché Sylviane S Graillot Vanessa V Levêque Mathilde M Bézirard Valérie V Héliès-Toussaint Cécile C Estrada Florence Blas Y FBY Tondereau Valérie V Lippi Yannick Y Naylies Claire C Peyriga Lindsey L Canlet Cécile C Davila Anne Marie AM Blachier François F Ferrier Laurent L Boutet-Robinet Elisa E Guéraud Françoise F Théodorou Vassilia V Pierre Fabrice H F FHF

Microbiome 20190506 1

<h4>Background</h4>The World Health Organization classified processed and red meat consumption as "carcinogenic" and "probably carcinogenic", respectively, to humans. Haem iron from meat plays a role in the promotion of colorectal cancer in rodent models, in association with enhanced luminal lipoperoxidation and subsequent formation of aldehydes. Here, we investigated the short-term effects of this haem-induced lipoperoxidation on mucosal and luminal gut homeostasis including microbiome in F344 ...[more]

PMID: 31060614

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Dataset Information

Haem iron reshapes colonic luminal environment: impact on mucosal homeostasis and microbiome through aldehyde formation.

Background

Results

Conclusions

Publications

Haem iron reshapes colonic luminal environment: impact on mucosal homeostasis and microbiome through aldehyde formation.

Similar Datasets

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