High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source.

ABSTRACT: Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives to scarce X-ray free-electron laser sources. Synchrotron radiation flux may be increased ?100 times by a moderate increase in the bandwidth ('pink beam' conditions) at some cost to data analysis complexity. Here, we report the first high-viscosity injector-based pink-beam SX experiments. The structures of proteinase K (PK) and A_2A adenosine receptor (A_2AAR) were determined to resolutions of 1.8 and 4.2?Å using 4 and 24 consecutive 100?ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A_2AAR data required an alternative data-processing strategy. This demonstration of the feasibility presents new opportunities for time-resolved experiments with microcrystals to study structural changes in real time at pink-beam synchrotron beamlines worldwide.

SUBMITTER: Martin-Garcia JM 

PROVIDER: S-EPMC6503920 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature