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Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant.

ABSTRACT: An atypical urease-negative mutant of Actinobacillus pleuropneumoniae serovar 2 was isolated in Japan. Nucleotide sequence analysis of the urease gene cluster revealed that the insertion of a short DNA sequence into the cbiM gene was responsible for the urease-negative activity of the mutant. Veterinary diagnostic laboratories should be watchful for the presence of aberrant urease-negative A. pleuropneumoniae isolates.

SUBMITTER: Ito H

PROVIDER: S-EPMC6504136 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature

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Isolation and molecular characterization of a urease-negative Actinobacillus pleuropneumoniae mutant.

Ito Hiroya H Takahashi Sayaka S Asai Tetsuo T Tamura Yutaka Y Yamamoto Koshi K

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 20171116 1

An atypical urease-negative mutant of Actinobacillus pleuropneumoniae serovar 2 was isolated in Japan. Nucleotide sequence analysis of the urease gene cluster revealed that the insertion of a short DNA sequence into the cbiM gene was responsible for the urease-negative activity of the mutant. Veterinary diagnostic laboratories should be watchful for the presence of aberrant urease-negative A. pleuropneumoniae isolates. ...[more]

PMID: 29145759

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Novel genes affecting urease acivity in Actinobacillus pleuropneumoniae.

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2011-11-09 | GSE19056 | GEO

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