Digital Image Analysis of BAP-1 Accurately Predicts Uveal Melanoma Metastasis.

ABSTRACT: Purpose:Reduced nuclear expression of BRCA1 associated protein 1 (BAP-1) is associated with a high risk for metastasis in uveal melanoma. Manual assessment of the expression level may face issues with interobserver reproducibility. This could be improved with digital image analysis (DIA). Methods:Thirty enucleated eyes with uveal melanoma from the Emory Eye Center (Atlanta, GA; years 2009-2017) were included and stained with BAP-1. Retrospective data on patient and tumor characteristics were retrieved. Patients were randomized to a training or validation cohort. Their tumor sections were digitally scanned and scored for percentage of BAP-1-positive cells with the QuPath Bioimage analysis software. Results:Interobserver concordance was 75% (Cohen's ? 0.52) with manual BAP-1 scoring and 88% to 94% with DIA (Cohen's ? 0.75-0.88). Positive and negative predictive values for metastasis were 90% and 100% with DIA, 80% and 86% with manual scoring, and 78% and 88% with gene expression class 2. In binary logistic regression, manual and DIA of BAP-1 and gene expression class 2 were associated with metastasis, but none retained significance in multiple regression. Metastasis-free survival was significantly shorter with low BAP-1 expression as defined by DIA (log-rank P = 0.02), but not with manual scoring (log-rank P = 0.36) or with gene expression class 2 (log-rank P = 0.17). Conclusions:DIA of BAP-1 is a competitive alternative to manual assessment as well as gene expression profiling in prognostication of enucleated specimens with uveal melanoma. Translational Relevance:The emerging scope for automatization of qualified diagnostic tasks is applied to uveal melanoma.

SUBMITTER: Stalhammar G 

PROVIDER: S-EPMC6504204 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature