Unknown

Dataset Information

0

HSB-1 Inhibition and HSF-1 Overexpression Trigger Overlapping Transcriptional Changes To Promote Longevity in Caenorhabditis elegans.


ABSTRACT: Heat shock factor 1 (HSF-1) is a component of the heat shock response pathway that is induced by cytoplasmic proteotoxic stress. In addition to its role in stress response, HSF-1 also acts as a key regulator of the rate of organismal aging. Overexpression of HSF-1 promotes longevity in C. elegans via mechanisms that remain less understood. Moreover, genetic ablation of a negative regulator of HSF-1, termed as heat shock factor binding protein 1 (HSB-1), results in hsf-1-dependent life span extension in animals. Here we show that in the absence of HSB-1, HSF-1 acquires increased DNA binding activity to its genomic target sequence. Using RNA-Seq to compare the gene expression profiles of the hsb-1 mutant and hsf-1 overexpression strains, we found that while more than 1,500 transcripts show ?1.5-fold upregulation due to HSF-1 overexpression, HSB-1 inhibition alters the expression of less than 500 genes in C. elegans Roughly half of the differentially regulated transcripts in the hsb-1 mutant have altered expression also in hsf-1 overexpressing animals, with a strongly correlated fold-expression pattern between the two strains. In addition, genes that are upregulated via both HSB-1 inhibition and HSF-1 overexpression include numerous DAF-16 targets that have known functions in longevity regulation. This study identifies how HSB-1 acts as a specific regulator of the transactivation potential of HSF-1 in non-stressed conditions, thus providing a detailed understanding of the role of HSB-1/HSF-1 signaling pathway in transcriptional regulation and longevity in C. elegans.

SUBMITTER: Sural S 

PROVIDER: S-EPMC6505166 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

altmetric image

Publications

HSB-1 Inhibition and HSF-1 Overexpression Trigger Overlapping Transcriptional Changes To Promote Longevity in <i>Caenorhabditis elegans</i>.

Sural Surojit S   Lu Tzu-Chiao TC   Jung Seung Ah SA   Hsu Ao-Lin AL  

G3 (Bethesda, Md.) 20190507 5


Heat shock factor 1 (HSF-1) is a component of the heat shock response pathway that is induced by cytoplasmic proteotoxic stress. In addition to its role in stress response, HSF-1 also acts as a key regulator of the rate of organismal aging. Overexpression of HSF-1 promotes longevity in <i>C. elegans</i> via mechanisms that remain less understood. Moreover, genetic ablation of a negative regulator of HSF-1, termed as heat shock factor binding protein 1 (HSB-1), results in <i>hsf-1</i>-dependent l  ...[more]

Similar Datasets

| S-EPMC7136021 | biostudies-literature
| S-EPMC2709959 | biostudies-other
| S-EPMC5570370 | biostudies-literature
2020-04-03 | GSE128935 | GEO
| S-EPMC3183074 | biostudies-literature
| S-EPMC1626236 | biostudies-literature
| S-EPMC1559758 | biostudies-literature
| S-EPMC5741582 | biostudies-literature
| S-EPMC5389855 | biostudies-literature
| S-EPMC3368121 | biostudies-literature