High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters.

ABSTRACT:

Background

Nattokinase (NK), which is a member of the subtilisin family, is a potent fibrinolytic enzyme that might be useful for thrombosis therapy. Extensive work has been done to improve its production for the food industry. The aim of our study was to enhance NK production by tandem promoters in Bacillus subtilis WB800.

Results

Six recombinant strains harboring different plasmids with a single promoter (P_P43, P_HpaII, P_BcaprE, P_gsiB, P_yxiE or P_luxS) were constructed, and the analysis of the fibrinolytic activity showed that P_P43 and P_HpaII exhibited a higher expression activity than that of the others. The NK yield that was mediated by P_P43 and P_HpaII reached 140.

SUBMITTER: Liu Z 

PROVIDER: S-EPMC6505213 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature