Dataset Information

Zika virus detection, isolation and genome sequencing through Culicidae sampling during the epidemic in Vitoria, Espirito Santo, Brazil.

ABSTRACT: BACKGROUND:Zika virus (ZIKV) has been isolated from many mosquito species in nature, but it is believed that the main vectors in urban environments are species of the genus Aedes. Here, we detected and isolated ZIKV in samples from Aedes aegypti, Aedes taeniorhynchus and Culex quinquefasciatus, collected during the Zika epidemic in Vitória, southeast Brazil. Using quantitative real-time polymerase chain reaction, ZIKV detection was performed in mosquito samples collected from February to April 2016. RESULTS:Overall, six pools of mosquitoes were positive for ZIKV: four of Cx. quinquefasciatus, one of Ae. aegypti and one of Ae. taeniorhynchus. Their genomes were sequenced. CONCLUSIONS:These results support and strengthen the hypothesis that other mosquito species can also be involved in ZIKV transmission.

SUBMITTER: Ayres CFJ

PROVIDER: S-EPMC6505216 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature

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Zika virus detection, isolation and genome sequencing through Culicidae sampling during the epidemic in Vitória, Espírito Santo, Brazil.

Ayres Constância Flavia Junqueira CFJ Guedes Duschinka Ribeiro Duarte DRD Paiva Marcelo Henrique Santos MHS Morais-Sobral Mariana Carolina MC Krokovsky Larissa L Machado Laís Ceschini LC Melo-Santos Maria Alice Varjal MAV Crespo Mônica M Oliveira Cláudia Maria Fontes CMF Ribeiro Ricardo Silva RS Cardoso Orlei Amaral OA Menezes Ana Lúcia Barbosa ALB Laperrière-Jr Roberto Costa RC Luna Carlos Feitosa CF Oliveira André Luiz Sá ALS Leal Walter Soares WS Wallau Gabriel Luz GL

Parasites & vectors 20190508 1

<h4>Background</h4>Zika virus (ZIKV) has been isolated from many mosquito species in nature, but it is believed that the main vectors in urban environments are species of the genus Aedes. Here, we detected and isolated ZIKV in samples from Aedes aegypti, Aedes taeniorhynchus and Culex quinquefasciatus, collected during the Zika epidemic in Vitória, southeast Brazil. Using quantitative real-time polymerase chain reaction, ZIKV detection was performed in mosquito samples collected from February to ...[more]

PMID: 31068218

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Dataset Information

Zika virus detection, isolation and genome sequencing through Culicidae sampling during the epidemic in Vitoria, Espirito Santo, Brazil.

Publications

Zika virus detection, isolation and genome sequencing through Culicidae sampling during the epidemic in Vitória, Espírito Santo, Brazil.

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