Live pigs produced from genome edited zygotes.
Ontology highlight
ABSTRACT:
SUBMITTER: Lillico SG
PROVIDER: S-EPMC6505673 | biostudies-literature | 2013 Oct
REPOSITORIES: biostudies-literature
Publications
Live pigs produced from genome edited zygotes.
Lillico Simon G SG Proudfoot Chris C Carlson Daniel F DF Stverakova Dana D Neil Claire C Blain Carol C King Tim J TJ Ritchie William A WA Tan Wenfang W Mileham Alan J AJ McLaren David G DG Fahrenkrug Scott C SC Whitelaw C Bruce A CB
Scientific reports 20131010
Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome. Here we demonstrate for the first time that both TALEN and ZFN injected directly into pig zygotes can produce live genome edited pigs. Monoallelic as well as heterozygous and homozygous biallelic events were identified, significantly broadening the use of genome editor technology in livestock by enabling gene knockout in zygotes from any ...[more]
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