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Development and validation of a real-time PCR specific for the leukotoxin gene of Bibersteinia trehalosi.

ABSTRACT: A real-time PCR assay for the leukotoxin gene of Bibersteinia trehalosi was developed and validated to better identify this pathogen, which is a cause of respiratory disease in bighorn sheep. The specificity of the PCR primers was evaluated with DNA from 59 known isolates of the Pasteurellaceae family. For validation, 162 field samples were compared using both the new assay and an indirect method using 2 sets of published protocols. The real-time PCR assay was found to be specific for the leukotoxin gene of B. trehalosi and provides a rapid and direct approach for detecting leukotoxin-producing forms of this organism from samples containing mixed species of leukotoxin-positive Pasteurellaceae.

SUBMITTER: Killion HJ

PROVIDER: S-EPMC6505900 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Development and validation of a real-time PCR specific for the leukotoxin gene of Bibersteinia trehalosi.

Killion Halcyon J HJ Edwards William W Jennings-Gaines Jessica J Wood Mary M Fox Karen K Sondgeroth Kerry K

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 20180330 4

A real-time PCR assay for the leukotoxin gene of Bibersteinia trehalosi was developed and validated to better identify this pathogen, which is a cause of respiratory disease in bighorn sheep. The specificity of the PCR primers was evaluated with DNA from 59 known isolates of the Pasteurellaceae family. For validation, 162 field samples were compared using both the new assay and an indirect method using 2 sets of published protocols. The real-time PCR assay was found to be specific for the leukot ...[more]

PMID: 29601780

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