Surface patches on recombinant erythropoietin predict protein solubility: engineering proteins to minimise aggregation.

ABSTRACT:

Background

Protein solubility characteristics are important determinants of success for recombinant proteins in relation to expression, purification, storage and administration. Escherichia coli offers a cost-efficient expression system. An important limitation, whether for biophysical studies or industrial-scale production, is the formation of insoluble protein aggregates in the cytoplasm. Several strategies have been implemented to improve soluble expression, ranging from modification of culture conditions to inclusion of solubility-enhancing tags.

Results

Surface patch analysis has been applied to predict amino acid changes that can alter the solubility of expressed recombinant human erythropoietin (rHuEPO) in E. coli, a factor that has importance for both yield and subse

SUBMITTER: Carballo-Amador MA 

PROVIDER: S-EPMC6507049 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature