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Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells.

ABSTRACT: A long wavelength TCF-based fluorescent probe (TCF-ALP) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of TCF-ALP resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. TCF-ALP was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, TCF-ALP proved capable of imaging endogenously stimulated ALP in myogenic murine C2C12 cells. Overall, TCF-ALP offers promise as an effective fluorescent/colorimetric probe for evaluating phosphatase activity in clinical assays or live cell systems.

SUBMITTER: Gwynne L 

PROVIDER: S-EPMC6508040 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Long Wavelength TCF-Based Fluorescent Probe for the Detection of Alkaline Phosphatase in Live Cells.

Gwynne Lauren L   Sedgwick Adam C AC   Gardiner Jordan E JE   Williams George T GT   Kim Gyoungmi G   Lowe John P JP   Maillard Jean-Yves JY   Jenkins A Toby A ATA   Bull Steven D SD   Sessler Jonathan L JL   Yoon Juyoung J   James Tony D TD  

Frontiers in chemistry 20190430

A long wavelength TCF-based fluorescent probe (<b>TCF-ALP</b>) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of <b>TCF-ALP</b> resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. <b>TCF-ALP</b> was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, <b>TCF-ALP</b> proved capable of imaging  ...[more]

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