Ontology highlight
ABSTRACT:
SUBMITTER: Gwynne L
PROVIDER: S-EPMC6508040 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Frontiers in chemistry 20190430
A long wavelength TCF-based fluorescent probe (<b>TCF-ALP</b>) was developed for the detection of alkaline phosphatase (ALP). ALP-mediated hydrolysis of the phosphate group of <b>TCF-ALP</b> resulted in a significant fluorescence "turn on" (58-fold), which was accompanied by a colorimetric response from yellow to purple. <b>TCF-ALP</b> was cell-permeable, which allowed it to be used to image ALP in HeLa cells. Upon addition of bone morphogenic protein 2, <b>TCF-ALP</b> proved capable of imaging ...[more]