MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market.
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ABSTRACT:
SUBMITTER: Fraiture MA
PROVIDER: S-EPMC6509135 | biostudies-literature | 2019 May
REPOSITORIES: biostudies-literature
Publications
MinION sequencing technology to characterize unauthorized GM petunia plants circulating on the European Union market.
Fraiture Marie-Alice MA Ujhelyi Gabriella G Ovesná Jaroslava J Van Geel Dirk D De Keersmaecker Sigrid S Saltykova Assia A Papazova Nina N Roosens Nancy H C NHC
Scientific reports 20190509 1
In order to characterize unauthorized genetically modified petunia, an integrated strategy has been applied here on several suspected petunia samples from the European market. More precisely, DNA fragments of interest were produced by DNA walking anchored on key targets, earlier detected by real-time PCR screening analysis, to be subsequently sequenced using the MinION platform from Oxford Nanopore Technologies. This way, the presence of genetically modified petunia was demonstrated via the char ...[more]
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