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IR-783 Labeling of a Peptide Receptor for 'Turn-On' Fluorescence Based Sensing.

ABSTRACT: In this study, we examine a means for developing near-IR fluorescent sensors through streamlined, site-specific coupling with peptide-based receptors. As the penultimate step of solid-phase synthesis of a peptide-based receptor, we show a simple means of labeling the N' terminus with the near IR fluorophore IR-783 to afford a viable fluorescent sensor after cleavage from the resin. The proof-of-concept probe utilized a biotin mimetic peptide sequence as the receptive moiety. Here we revealed a "turn-on" fluorescence enhancement upon binding of the biotin mimetic probe to its intended streptavidin target. Not all peptide-receptive moieties tested were able to generate such an enhancement upon target binding, and as such, the rationale for the observed fluorescence response properties is discussed.

SUBMITTER: Kwak EA

PROVIDER: S-EPMC6510487 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

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IR-783 Labeling of a Peptide Receptor for 'Turn-On' Fluorescence Based Sensing.

Kwak Eun-A EA Kydd LeNaiya L Lim Butaek B Jaworski Justyn J

Chemosensors (Basel, Switzerland) 20181016 4

In this study, we examine a means for developing near-IR fluorescent sensors through streamlined, site-specific coupling with peptide-based receptors. As the penultimate step of solid-phase synthesis of a peptide-based receptor, we show a simple means of labeling the N' terminus with the near IR fluorophore IR-783 to afford a viable fluorescent sensor after cleavage from the resin. The proof-of-concept probe utilized a biotin mimetic peptide sequence as the receptive moiety. Here we revealed a " ...[more]

PMID: 31080779

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