Dataset Information

Histopathological and Molecular Evaluation of the Experimentally Infected Goats by the Larval Forms of Taenia multiceps.

ABSTRACT: Background:Introduction of Taenia multiceps and T. gaigeri as two separate species have been recognized mainly on morphological grounds. This experimental study was conducted to test whether cerebral and non-cerebral forms of Coenurus cerebralis belong to one origin or they are originated from two different tape worms. Methods:Two groups of dogs were infected with the cerebral and muscular sources of the coenuri cysts. About two months later the eggs were collected from the fecal samples to be used to experimentally infect other healthy goats. Histopathological and molecular evaluation was conducted in two groups of goats that were challenged with T. multiceps eggs obtained from the infected dogs by brain and muscular sources of coenuri cysts in School of Veterinary Medicine of Shiraz University, Shiraz, Iran in 2015. All aberrant sites of predilection of the metacestode in goats were muscles, heart, diaphragm and lungs. The brain and spinal cord were carefully dissected and examined but the cysts were not found in these locations. In addition, the molecular genetic markers of mitochondrial DNA (CO1 and ND1) were applied to resolve the questionable relationship between T. multiceps and T. gaigeri. Results:The larval stages of T. multiceps in brain and in other aberrant sites, which showed similar morphological criteria, were monophyletic species. Conclusion:Therefore, T. gaigeri must be considered taxonomically invalid.

SUBMITTER: Amrabadi O

PROVIDER: S-EPMC6511590 | biostudies-literature | 2019 Jan-Mar

REPOSITORIES: biostudies-literature

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Publications

Histopathological and Molecular Evaluation of the Experimentally Infected Goats by the Larval Forms of <i>Taenia multiceps</i>.

Amrabadi Omidreza O Oryan Ahmad A Moazeni Mohammad M Shari-Fiyazdi Hassan H Akbari Maryam M

Iranian journal of parasitology 20190101 1

<h4>Background</h4>Introduction of <i>Taenia multiceps</i> and <i>T. gaigeri</i> as two separate species have been recognized mainly on morphological grounds. This experimental study was conducted to test whether cerebral and non-cerebral forms of <i>Coenurus cerebralis</i> belong to one origin or they are originated from two different tape worms.<h4>Methods</h4>Two groups of dogs were infected with the cerebral and muscular sources of the coenuri cysts. About two months later the eggs were coll ...[more]

PMID: 31123473

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Project description:BackgroundThe larval stage of Taenia multiceps, a global cestode, encysts in the central nervous system (CNS) of sheep and other livestock. This frequently leads to their death and huge socioeconomic losses, especially in developing countries. This parasite can also cause zoonotic infections in humans, but has been largely neglected due to a lack of diagnostic techniques and studies. Recent developments in next-generation sequencing provide an opportunity to explore the transcriptome of T. multiceps.Methodology/principal findingsWe obtained a total of 31,282 unigenes (mean length 920 bp) using Illumina paired-end sequencing technology and a new Trinity de novo assembler without a referenced genome. Individual transcription molecules were determined by sequence-based annotations and/or domain-based annotations against public databases (Nr, UniprotKB/Swiss-Prot, COG, KEGG, UniProtKB/TrEMBL, InterPro and Pfam). We identified 26,110 (83.47%) unigenes and inferred 20,896 (66.8%) coding sequences (CDS). Further comparative transcripts analysis with other cestodes (Taenia pisiformis, Taenia solium, Echincoccus granulosus and Echincoccus multilocularis) and intestinal parasites (Trichinella spiralis, Ancylostoma caninum and Ascaris suum) showed that 5,100 common genes were shared among three Taenia tapeworms, 261 conserved genes were detected among five Taeniidae cestodes, and 109 common genes were found in four zoonotic intestinal parasites. Some of the common genes were genes required for parasite survival, involved in parasite-host interactions. In addition, we amplified two full-length CDS of unigenes from the common genes using RT-PCR.Conclusions/significanceThis study provides an extensive transcriptome of the adult stage of T. multiceps, and demonstrates that comparative transcriptomic investigations deserve to be further studied. This transcriptome dataset forms a substantial public information platform to achieve a fundamental understanding of the biology of T. multiceps, and helps in the identification of drug targets and parasite-host interaction studies.

Dataset Information

Histopathological and Molecular Evaluation of the Experimentally Infected Goats by the Larval Forms of Taenia multiceps.

Publications

Histopathological and Molecular Evaluation of the Experimentally Infected Goats by the Larval Forms of <i>Taenia multiceps</i>.

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