High-dimensional immune phenotyping and transcriptional analyses reveal robust recovery of viable human immune and epithelial cells from frozen gastrointestinal tissue.
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ABSTRACT:
SUBMITTER: Konnikova L
PROVIDER: S-EPMC6512331 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Publications
High-dimensional immune phenotyping and transcriptional analyses reveal robust recovery of viable human immune and epithelial cells from frozen gastrointestinal tissue.
Konnikova Liza L Boschetti Gilles G Rahman Adeeb A Mitsialis Vanessa V Lord James J Richmond Camilla C Tomov Vesselin T VT Gordon Will W Jelinsky Scott S Canavan James J Liss Andrew A Wall Sarah S Field Michael M Zhou Fanny F Goldsmith Jeffery D JD Bewtra Meenakshi M Breault David T DT Merad Miriam M Snapper Scott B SB
Mucosal immunology 20180815 6
Simultaneous analyses of peripheral and mucosal immune compartments can yield insight into the pathogenesis of mucosal-associated diseases. Although methods to preserve peripheral immune cells are well established, studies involving mucosal immune cells have been hampered by lack of simple storage techniques. We provide a cryopreservation protocol allowing for storage of gastrointestinal (GI) tissue with preservation of viability and functionality of both immune and epithelial cells. These metho ...[more]
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